Pathological findings by fixative time and formulation for Porites evermanni and Montipora capitata

Collections for histopathology in corals require holding of samples in seawater for a time before they are fixed for histopathology processing. This could adversely affect interpretation of morphologic changes during histopathology examinations. We evaluated microscopic morphology of Porites evermanni and Montipora capitata, held (0-120 min) in seawater prior to fixation in Z-Fix formulated with raw or artificial seawater. We saw no evident effects of treatments on microscopic morphology. However, among 88 statistical comparisons, and after accounting for false discovery rate, holding time prior to fixation was associated with a significant increase in degree of mucosity of basal body wall.

珊瑚组织病理学研究所需的样本需先在海水中保存一段时间，再进行固定以开展组织病理学处理。该前置保存步骤可能会对组织病理学检查中的形态学变化判读造成不利影响。本研究针对澄黄滨珊瑚（Porites evermanni）与多孔鹿角珊瑚（Montipora capitata）的样本展开评估：将其在海水中浸泡0至120分钟后，分别使用添加天然海水或人工海水的Z-Fix固定液进行固定，随后观察其显微形态。结果显示，各处理组对样本显微形态无显著影响。不过，在完成88项统计学比较并经错误发现率（false discovery rate）校正后，样本固定前的海水浸泡时长与基底体壁的黏液程度显著升高存在显著关联。