Molecular signatures of in-situ to invasive progression for basal-like breast cancers: An integrated mouse model and human tumor study [mouse]

This study identifies progression in breast ductal carcinoma in situ (DCIS) as it progresses towards triple negative invasive breast cancer (TNBC). Overall design: Single cell RNA sequencing (scRNAseq) and bulk RNA sequencing was performed on the C3Tag genetically engineered mouse model that forms human breast-like DCIS and TNBC. Bulk RNA sequencing was performed on RNA harvested from 10 archival formalin-fixed paraffin embedded (FFPE) pairs comprising of estrogen receptor (ER) positive (ER+) and ER- subsets of DCIS.

本研究聚焦乳腺导管原位癌（breast ductal carcinoma in situ, DCIS）向三阴性浸润性乳腺癌（triple negative invasive breast cancer, TNBC）的进展过程。实验总体设计如下：采用可构建类人乳腺DCIS与TNBC模型的C3Tag基因工程小鼠，开展单细胞RNA测序（single cell RNA sequencing, scRNAseq）与批量RNA测序（bulk RNA sequencing）；同时对10对存档福尔马林固定石蜡包埋（archival formalin-fixed paraffin embedded, FFPE）样本进行研究，该批样本涵盖雌激素受体阳性（ER+）与雌激素受体阴性（ER-）两类DCIS亚组，对其提取的RNA开展批量RNA测序。