Detection of plasmid-mediated AmpC-producers by eazyplex® SuperBug AmpC assay compared to whole-genome sequencing

eazyplex® SuperBug AmpC assay was evaluated on 71 Enterobacterales with phenotypically confirmed AmpC production. Sensitivity and specificity for detection of most common plasmidic ampC (pampC) types CMY and DHA was 100%. This assay proved useful for rapid pampC confirmation in Klebsiella spp. and Proteus spp. and distinction between pampC and chromosomally encoded ampC in E. coli.

本研究对eazyplex® SuperBug AmpC检测试剂盒（eazyplex® SuperBug AmpC assay）开展评估，共纳入71株经表型确认产AmpC酶的肠杆菌目（Enterobacterales）菌株。该试剂盒针对最常见的两种质粒型ampC（plasmidic ampC，pampC）亚型CMY与DHA的检测灵敏度及特异性均为100%。实验结果证实，该试剂盒可用于克雷伯菌属（Klebsiella spp.）与变形杆菌属（Proteus spp.）的质粒型ampC快速确证，同时可区分大肠杆菌（E. coli）中的质粒型ampC与染色体编码型ampC。