Expression profiling of secondary wild type (WT) and Ezh2-null murine MLL-AF9 AML. Mus musculus

We evaluated gene expression changes in secondary recipient murine leukemia caused by retroviral overexpression of MLL-AF9. We compared wild-type (WT) leukemia cells with mutant leukemia cells after cre-mediated inactivation of a homozygous conditional allele for Ezh2, a component of the Polycomb Repressive Complex2. Overall design: For WT cells, 4 biological replicates were hybridized. For Ezh2-null cells, 5 biological replicates were hybridized.本研究评估了由MLL-AF9逆转录病毒过表达诱导的继发性受体小鼠白血病中的基因表达变化。我们将野生型（WT）白血病细胞与经Cre重组酶介导的Ezh2纯合条件性等位基因失活后获得的突变白血病细胞进行了比较，其中Ezh2是多梳抑制复合体2（Polycomb Repressive Complex2）的组成亚基。实验整体设计：野生型细胞组设置4个生物学重复并完成杂交检测；Ezh2缺失细胞组设置5个生物学重复并完成杂交检测。