Proteomic analysis of cucumber (Cucumis sativus) fruit peel in response to pre-storage cold acclimation

To distinguish PsCA-responsive proteins, total proteins extracted from the non-acclimated fruit, the fruit exposed to 3 d of cold acclimation, the control fruit stored at 5oC for 12 d and the fruit exposed to 3d of PsCA treatment followed by 9 d of cold storage were separated by 2-DE, respectively. More than 750 protein spots were detected using PDQuest Advanced 2-D Gel Analysis software (Version 8.0) following the 2-DE gel analysis. Of the 750 spots, 58 that displayed significant and reproducible changes in abundance were isolated, and 56 spots were successfully characterized using MS/MS coupled with database searching, which matched 51 proteins. 38 proteins have scores greater than threshold scores of 61(P < 0.05) and they are regarded as the significant differentially- accumulated proteins (SDAPs).

为鉴定响应PsCA的蛋白质（PsCA-responsive proteins），本实验分别从非驯化果实、经3天低温驯化的果实、于5℃储存12天的对照果实，以及经3天PsCA处理后再进行9天低温储存的果实中提取总蛋白，采用双向凝胶电泳（two-dimensional gel electrophoresis，2-DE）进行分离。经双向凝胶电泳分析后，使用PDQuest Advanced二维凝胶分析软件（版本8.0）共检测到750余个蛋白点。在这750个蛋白点中，有58个蛋白点的丰度呈现出显著且可重复的变化，对其进行分离后，通过串联质谱（MS/MS）结合数据库检索，成功鉴定了56个蛋白点，对应51种蛋白质。其中38种蛋白质的得分高于61的阈值得分（P < 0.05），被认定为显著差异累积蛋白质（SDAPs）。