The proteome of Synechocystis sp. PCC 6803 cells grown under light-activated heterotrophic growth

Cyanobacteria are photoautotrophic prokaryotes with a plant-like photosynthetic machinery. Besides being able to grow photoautotrophically, some cyanobacteria are also capable to grow photoheterotrophically, where they use reduced organic compounds as carbon source, or even completely heterotrophically by using reduced organic compounds as carbon and energy source. The well characterized cyanobacterium Synechocystis sp. PCC 6803 can grow in darkness under light-activated heterotrophic growth (LAHG) conditions by using glucose as carbon and energy source. In the present work, we combined pre-fractioning of Synechocystis cellular membranes with a global proteome and lipidome analysis, to shift the analytical focus towards the rearrangement of the internal thylakoid membrane system observed in Synechocystis cells under LAHG conditions.

蓝细菌（Cyanobacteria）是一类拥有类植物光合装置的光能自养原核生物。除可通过光能自养方式生长外，部分蓝细菌还可采用光能异养路径，以还原态有机化合物作为碳源进行增殖；甚至有部分蓝细菌可完全以异养方式存活，将还原态有机化合物同时作为碳源与能量来源。已被充分表征的集胞藻属PCC 6803（Synechocystis sp. PCC 6803），可在光激活异养生长（light-activated heterotrophic growth, LAHG）条件下，以葡萄糖作为碳源与能量源在黑暗环境中生长。本研究将集胞藻细胞膜的预分级处理与全局蛋白质组、脂质组分析相结合，旨在将分析重点聚焦于LAHG条件下集胞藻细胞内观察到的内部类囊体膜系统重排现象。