Comparative intraindividual transcriptome analysis of B-precursor ALL of childhood. Homo sapiens

The objective of this study was the assessment of transcriptional dysregulation in particular with regard to B-cell differentiation factors. Most studies focus on cross-section analyses of various leukemia subtypes to identify differentially regulated genes lacking suitable reference models. Here we applied comparative intraindividual transcriptome analysis of B-precursor ALL of childhood, which introduces a side-by-side analysis of leukemic cells and matched normal lymphoblasts from the same individual in complete continuous remission after the end of re-induction therapy. This approach reduces noise by eliminating interindividual variability. Overall design: Comparative matched pair analysis of (single sorted) initial leukemia and single sorted lymphoblasts isolated from the same individual, n=4 pairs, reference samples are the single sorted normal lymphoblasts. Please note that there are sorted samples form only three of the patients. Due to experimental circumstances, a "sorted" sample from the last patient was not included.

本研究旨在评估转录失调情况，尤其聚焦于B细胞分化相关因子。既往多数研究聚焦于各类白血病亚型的横断面分析，以识别差异调控基因，但此类研究均缺乏合适的参照模型。本研究针对儿童B系前体急性淋巴细胞白血病（B-precursor ALL），采用个体内比较转录组分析策略，对同一患者经再诱导治疗结束后达到完全持续缓解状态时的白血病细胞与匹配的正常淋巴母细胞开展同步对比分析。该方案通过消除个体间差异，有效降低实验噪声。总体实验设计：对来自同一患者的（单分选）初始白血病细胞与单分选淋巴母细胞进行配对比较分析，共纳入4对样本，参照样本为单分选正常淋巴母细胞。需注意，仅3例患者获取了分选样本。受实验条件限制，最后1例患者的"分选"样本未被纳入本研究。