Neurodegenerative disease-linked microRNAs acting as signaling molecules modulate CNS neuron structure and viability

The goal of this study is to compare gene expression of mouse cultured cortical neurons following exposure to extracellular miR-124-5p, and miR-92a-1-5p, which are dysregulated in Alzheimer´s disease. Overall design: To investigate the effect of extracellular miRNAs on neurons, we exposed mouse cortical neurons to miR-92a-1-5p, miR-124-5p and a mutated control oligonucleotide, Mut.oligo, for 6h and 5d. Total RNA was extracted for RNA-seq, in 4 replicates, and comparative gene expression profiles were analyzed.

本研究旨在比较暴露于细胞外微小RNA-124-5p（miR-124-5p）与微小RNA-92a-1-5p（miR-92a-1-5p）后的小鼠培养皮层神经元的基因表达水平，这两种微小RNA在阿尔茨海默病中存在表达失调情况。
实验设计概述：为探究细胞外微小RNA对神经元的影响，我们将小鼠皮层神经元分别暴露于miR-92a-1-5p、miR-124-5p及突变型对照寡核苷酸Mut.oligo中，处理时长为6小时与5天。随后提取总RNA进行RNA测序（RNA-seq），实验设置4次重复，并对比较基因表达谱展开分析。