Impact of transcription factor ETV1 on HCT116 colon cancer cells.. Impact of transcription factor ETV1 on HCT116 colon cancer cells.

We identified that ETV1 downregulation affects growth of HCT116 cells, and concomitantly affects their transcriptome. This suggests that ETV1 might be involved in colon cancer formation and/or progression. Overall design: HCT116 cells were infected with retrovirus expressing control shRNA or two different ETV1 shRNAs (#1 and #5). RNA was extracted and subjected to RNA-sequencing.

我们发现ETV1下调可影响HCT116细胞的增殖活性，并同时改变其转录组表达谱。该结果提示ETV1可能参与结肠癌的发生与进展过程。实验设计：将HCT116细胞分别感染携带对照短发夹RNA（short hairpin RNA, shRNA）以及两种不同ETV1靶向短发夹RNA（#1与#5）的逆转录病毒。提取细胞总RNA后进行RNA测序（RNA-sequencing）分析。