Metabolomics to elucidate the effects of novel sustainable feed additives in the feed of farmed salmon (HoloFood Trial A - seaweed-dose response)

Testing the effect of blue mussel ensilage as a novel cost-efficient protein feed source for Atlantic salmon feed. Fermentation of novel raw materials for fish feed is a new practice with little prior experience of direct relevance to aquaculture and fish farming. In Trial A, an alternative marine compound is included in the feed, which is based on seaweed. In the trial were multiple inclusion levels used to investigate the effect of seaweed inclusion in an aquaculture setting.

HoloFood Trial A - seaweed-dose response is reported in the current study MTBLS4381.
HoloFood Trial B - blue mussel-dose response is reported in MTBLS4382.
HoloFood Trial C - blue mussel ensilage-dose response is reported in MTBLS4384.
HoloFood Trial D - fermented seaweed open water-dose response is reported in MTBLS6733.
HoloFood Trial 1/2/3 is reported in MTBLS6988.

Linked cross omic data sets:
Nucleic acid data associated with this study are available in the European Nucleotide Archive (ENA): accession number PRJEB43192.
Metagenomic data associated with this study are available from MGnify under the Super Study 'holofood'.

本研究旨在评估蓝贻贝青贮（blue mussel ensilage）作为大西洋鲑（Atlantic salmon）饲料新型低成本蛋白源的应用效果。鱼类饲料新型原料的发酵工艺尚属新兴实践，目前与水产养殖及鱼类繁育直接相关的前期经验较为匮乏。试验A中，饲料添加了一种以海藻为基底的替代水产饲料复合原料；本试验设置了多个添加梯度，以探究水产养殖体系中海藻添加的影响。

HoloFood试验A——海藻剂量响应试验的相关结果已在本研究MTBLS4381中报道；HoloFood试验B——蓝贻贝剂量响应试验的相关结果已发表于MTBLS4382；HoloFood试验C——蓝贻贝青贮剂量响应试验的相关结果已发表于MTBLS4384；HoloFood试验D——发酵海藻开放水域剂量响应试验的相关结果已发表于MTBLS6733；HoloFood试验1/2/3的相关结果已发表于MTBLS6988。

关联多组学数据集：
本研究相关的核酸数据可于欧洲核酸档案库（European Nucleotide Archive, ENA）获取，登录号为PRJEB43192。
本研究相关的宏基因组数据可从MGnify平台的超级研究项目'holofood'中获取。