Analysis of gene expression profiles of 48 ABC transporters in normal colon and colon cancer tissues

Description We aim to identify the expression levels of 48 ABC transporters comprehensively in normal colon and colon cancer tissues and compared expression profiles between those for elucidating mechanisms which regulate the expression of ABC transporters related anticancer drugs resistance. We sampled tumor tissues and corresponding normal tissues from 3 colon cancer patients who were underwent surgical resection of primary tumor without neoadjuvant chemotherapy in Tohoku University Hospital. Patient 1 is a 69 years old male had UICC stage IIIB ascending colon cancer. Patient 2 is a 61 year old male had UICC stage IIA ascending colon cancer. Patient 3 is a 56 year old female had UICC stage I sigmoid colon cancer. The expression levels of five colon cancer cell lines (COLO205, HCT116, HCT15, HT29, and SW620) registered in NCI60 were also analyzed.

数据集描述：本研究旨在全面鉴定正常结肠组织与结肠癌组织中48种ABC转运蛋白（ABC transporter）的表达水平，并对比二者的表达谱，以阐明调控与抗癌药物耐药相关的ABC转运蛋白表达的分子机制。本研究从东北大学附属医院收治的3例未接受新辅助化疗、仅行原发肿瘤切除术的结肠癌患者中采集肿瘤组织及其配对正常组织：患者1为69岁男性，罹患UICC分期IIIB期升结肠癌；患者2为61岁男性，罹患UICC分期IIA期升结肠癌；患者3为56岁女性，罹患UICC分期I期乙状结肠癌。本研究同时对NCI60数据库收录的5株结肠癌细胞系（COLO205、HCT116、HCT15、HT29及SW620）的表达水平进行了分析。