Functional and Molecular Single-Cell Analyses Implicate PRDM14 in the Initiation of a Novel B-1 Cell Malignancy - Cut and Run experiments. Functional and Molecular Single-Cell Analyses Implicate PRDM14 in the Initiation of a Novel B-1 Cell Malignancy - Cut and Run experiments

Molecular mechanisms underlying high-risk subtypes of leukemia remain elusive. PR domain-containing 14 (Prdm14) is a potent oncogene implicated in the initiation of many cancers, including leukemia. Here, we interrogate the heterogeneity of Prdm14-expressing cell types in a mouse model of T-cell acute lymphoblastic leukemia (T-ALL). We identified an abnormal B-1 cell-like population in pre-leukemic bone marrow. B-1 cells are a self-renewing population of unconventional B cells established during embryonic development. This dataset contains genome-wide profiling of PRDM14, H3K4me1, and H3K4me3 to understand the genome-wide binding of the key TF in the abnormal B1 population and their B-cell conserved and B-1 cell-type specific enhancer and promoter regions. Cut-and-run experiments for each antibody were completed in triplicate, with IgG included as an extra experimental control.

高危亚型白血病的潜在分子机制仍有待阐明。含PR结构域蛋白14（PR domain-containing 14，Prdm14）是一种强效致癌基因，其与包括白血病在内的多种癌症的发生密切相关。本研究针对T细胞急性淋巴细胞白血病（T-cell acute lymphoblastic leukemia，T-ALL）小鼠模型中表达Prdm14的细胞类型异质性展开解析。我们在白血病前期骨髓中鉴定出一类异常的B-1细胞样细胞群。B-1细胞是一类在胚胎发育阶段形成的、具有自我更新能力的非常规B细胞群体。本数据集包含PRDM14、H3K4me1及H3K4me3的全基因组谱分析数据，用于解析该关键转录因子（transcription factor，TF）在异常B-1细胞群中的全基因组结合特征，以及这些结合位点所处的B细胞保守型与B-1细胞特异性增强子和启动子区域。针对每种抗体的切割-运行（Cut-and-run）实验均完成了三次生物学重复，并额外设置IgG作为实验对照。