Gene expression profile at single cell level of lungs of adult male and female C57BL/6 mice with andwithout high-fat diet-induced obesity

Obesity is associated with various lung pathologies. We performed single nucleus RNA sequencing of formalin-fixed lungs of mice to obtain insights on this. Overall design: Groups of male and female mice of about 6 months age had been kept on ad libitum for about 3 months on high-fat or control diet (Bioserv products S3282 and F4031, with 60% and 10% fat calories respectively), when they were sacrificed with carbon dioxide-based euthanasia. Lungs were immediately dissected, rinsed in PBS, and the left lower lung lobes flash-frozen in liquid nitrogen. Nuclei isolated from frozen tissues were used for sequencing.

肥胖与多种肺部病理状态存在显著关联。为阐明二者间的潜在关联机制，我们针对小鼠福尔马林固定肺部组织完成了单细胞核RNA测序（single nucleus RNA sequencing）实验。
实验设计概况：将约6月龄的雌雄小鼠分为两组，分别以高脂饲料与对照饲料自由采食（ad libitum）饲养约3个月，两种饲料均购自Bioserv，产品编号分别为S3282与F4031，脂肪供能占比分别为60%与10%；随后采用二氧化碳安乐死法处死小鼠。解剖获取肺部组织后，立即用磷酸盐缓冲液（Phosphate Buffered Saline, PBS）漂洗，将左肺下叶置于液氮中快速冷冻。从上述冷冻组织中分离得到的细胞核将用于后续测序实验。