Rachel Horowitz-Scherer, Chris Woodcock (2010) CIL:777, Patiria miniata, sperm. CIL. Dataset

Phosphorus has a characteristic electron energy loss with an edge at 160 eV. The Zeiss 902 electron microscope permits images to be recorded at different energy losses which can be digitized and processed to yield a net phosphorus image. For nuclear chromatin, phosphorus is primarily located in DNA, allowing its distribution in chromatin fibers to be mapped. Electron energy loss (EELS) imaging is also known as electron spectroscopic imaging (ESI) and electron energy filtering TEM (EFTEM).
Starfish sperm chromatin, which has clearly defined chromatin fibers was fixed, embedded in Lowicryl at low temperature, thin sections prepared and imaged unstained. The 4 grouped images were recorded with a Zeiss 902 energy filtered TEM at 80KV using 5eV windows centered on energy loss values of 160, 170, 120 (this image), and 110eV.

磷元素在160电子伏特处表现出独特的电子能量损失特征。蔡司902型电子显微镜允许记录不同能量损失的图像，这些图像可以被数字化并处理，以生成净磷图像。对于核染色质，磷主要位于DNA中，这使得其在染色质纤维中的分布可以被定位。电子能量损失成像（EELS）也被称为电子能谱成像（ESI）和电子能量过滤透射电子显微镜（EFTEM）。海星精子染色质，其染色质纤维结构清晰，经过固定、低温Lowicryl包埋后制备了薄片，并进行了无染色成像。四组图像使用蔡司902型能量过滤透射电子显微镜在80KV电压下，利用以160、170、120（本图像）和110电子伏特能量损失值为中心的5电子伏特窗口进行记录。