SH1038829.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE数据库为界定、识别、传播及处理基于DNA的物种假说（DNA-based Species Hypotheses，SH）提供了统一的方法。国际核苷酸序列数据库中收录的所有真菌ITS（Internal Transcribed Spacer，内转录间隔区）序列，均通过应用一组动态距离阈值（0.5%~3.0%）被聚类至近似物种水平。每一条物种假说均会被赋予一个以数字对象标识符（DOI，Digital Object Identifier）为形式的唯一且稳定的名称，其分类学与生态学注释将通过分布式网络第三方注释工作完成核验。通过整合某一物种假说内所有序列的鉴定信息，尽可能将该物种假说与对应分类单元名称及其所属分类层级（如门、纲、目等）进行关联。随后，将选取一条自动或人工指定的序列作为该物种假说的代表序列。此类代表序列已发布于https://unite.ut.ee/repository.php，可供科研群体用于局部序列相似性搜索、下一代测序分析流程等研究场景。随着公共真菌ITS序列的数量持续增长，该系统及配套数据将自动完成更新。