Genome-wide analysis of parental wild-type and derived doxorubicin resistant SK-N-SH neuroblastoma cell lines as well as those co-treated with the histone deacetylase inhibitor SAHA

Analysis of varied biologic pathways in neuroblastoma SK-N-SH cells grown in doxorubicin and/or SAHA. The hypothesis was that SK-N-SH cells undergo a mesenchymal change through mesenchymal change resulting in a more invasive as well as drug resistant phenotype, and that the mechanism of SAHAs effect on drug resistance may be revealed through this analysis. Total RNA was isolated from wild type, SAHA treated wild type cells, doxorubicin resistant, and SAHA treated doxorubicin resistant SK-N-SH cell lines in triplicate.

本研究针对经阿霉素（doxorubicin）和/或SAHA处理培养的神经母细胞瘤（neuroblastoma）SK-N-SH细胞内的多种生物学通路展开分析。本研究提出如下假说：SK-N-SH细胞可通过间质转化（mesenchymal change）发生表型转变，获得更强的侵袭能力与药物耐药性；且通过本分析可揭示SAHA调控药物耐药性的分子机制。研究人员从四组SK-N-SH细胞系中分离总RNA，每组均设置三次生物学重复：野生型细胞、经SAHA处理的野生型细胞、阿霉素耐药型细胞、经SAHA处理的阿霉素耐药型细胞。