The miR-22-5p/Clec4e axis has diagnostic potential in fructose-induced nonalcoholic fatty liver disease

Nonalcoholic fatty liver disease (NAFLD) is the most common liver disease.MicroRNAs play roles in the onset and progression of the disease. This study aimed to screen microRNA profiles and potential RNA networks for the diagnosis and treatment of NAFLD. Mice were high-fructose diet (HFrD) fed to induce NAFLD. MicroRNA expression profiles of the livers in HFrD mice and chow-diet fed mice were analyzed by RNA-seq. We successfully constructed high fructose induced NAFLD. There are 13 differentially expressed (DE) miRNAs in the livers of NAFLD mice. In summary, this study furthered our understanding of the genome mechanisms and the development of potential biomarkers for the treatment of fructose-induced NAFLD. Male C57BL/6J mice aged 10-11 weeks were purchased from the Shanghai Laboratory Animal Company (SLAC, Shanghai, China). HFrD containing 10% kcal from fat, 70% kcal from carbohydrate, and 20% kcal from protein were purchased from Research Diets (D08040107, Research Diets, USA). After a 2-week intervention period in which mice were fed either a normal diet (ND) or HFrD, mice were anesthetized with 10% chloral hydrate.

非酒精性脂肪性肝病（Nonalcoholic fatty liver disease, NAFLD）是临床最为常见的肝脏疾病。微小RNA（microRNA）在该病的发生与进展过程中发挥关键调控作用。本研究旨在筛选可用于非酒精性脂肪性肝病诊断与治疗的microRNA表达谱及潜在RNA调控网络。研究通过高果糖饮食（high-fructose diet, HFrD）喂养小鼠，成功构建非酒精性脂肪性肝病动物模型；采用RNA测序（RNA-seq）分析高果糖饮食喂养小鼠与普通饲料喂养小鼠的肝脏microRNA表达谱。本研究在NAFLD小鼠肝脏中共鉴定出13个差异表达（differentially expressed, DE）microRNA。综上，本研究加深了我们对果糖诱导型非酒精性脂肪性肝病基因组调控机制的理解，为该病的治疗性潜在生物标志物开发提供了理论支撑。本研究选用10~11周龄的雄性C57BL/6J小鼠，购自上海斯莱克实验动物有限责任公司（Shanghai Laboratory Animal Company, SLAC，中国上海）。本研究使用的高果糖饲料热量配比为脂肪供能10%、碳水化合物供能70%、蛋白质供能20%，购自美国Research Diets公司（货号D08040107）。小鼠在接受为期2周的普通饲料（normal diet, ND）或高果糖饲料喂养干预后，采用10%水合氯醛进行麻醉。