Next generation sequencing analysis of gastric cancer

Gastric carcinoma tissues were obtained from 31 patients treated by surgical resection. The patients were subdivided into two groups according to the presence of peritoneal involvement, either macroscopically (P+) or microscopically (Cy+) at the time of the surgery: 19 patients had no peritoneal involvement at the time of diagnosis (P0 and Cy0) while 12 had peritoneal involvement, (P+ or Cy+). High-quality RNA was extracted from tumor gastric mucosa and healthy mucosa. Sequencing was performed on an Ion S5™ Sequencer with Torrent Suite™ Software v6 (Thermo Fisher Scientific). The analyses were performed with a range of fold <−2 and >+2 and a p value < 0.05, using Transcriptome Analysis Console Software (version 4.0.2), certified for AmpliSeq analysis.

本数据集的样本取自31例接受手术切除治疗的胃癌患者。根据手术时是否存在腹膜受累，将患者分为两组：其中19例患者诊断时无腹膜受累（P0及Cy0），剩余12例存在肉眼可见（P+）或镜下可见（Cy+）的腹膜受累。从肿瘤胃黏膜及健康胃黏膜中提取高质量核糖核酸（RNA），随后使用搭载Torrent Suite™软件v6的Ion S5™测序仪（Ion S5™ Sequencer）完成测序，该测序平台由赛默飞世尔科技（Thermo Fisher Scientific）提供。数据分析采用经AmpliSeq分析认证的转录组分析控制台软件（Transcriptome Analysis Console Software）v4.0.2完成，筛选标准为差异倍数<−2 或 >+2，且p值<0.05。