human skin microbiome of healthy children during puberty. human skin bacteria and fungi

Previous cross-sectional studies have shown that skin microbiomes in adults are distinct from children. However, the human skin microbiome in individuals as they sexually mature has not been studied as extensively. We performed a prospective, longitudinal study to investigate puberty-associated shifts in skin microbiota. Twelve healthy children were evaluated every 6-18 months for up to 6 years. Using 16S rRNA (V1-V3) and ITS1 amplicon sequencing analyzed with DADA2, we characterized the bacterial and fungal communities of 5 different skin and nares sites.

既往横断面研究已证实，成人皮肤微生物组与儿童存在显著差异。然而，针对个体性成熟阶段的人体皮肤微生物组，相关研究尚未得到充分开展。本研究开展了一项前瞻性纵向研究，以探究皮肤微生物群与青春期相关的动态变化。研究共纳入12名健康儿童，对其进行长达6年、每6至18个月一次的随访评估。本研究采用16S核糖体RNA（V1-V3区）及内转录间隔区1（ITS1）扩增子测序技术，并通过DADA2工具进行数据分析，对5处不同皮肤及鼻孔位点的细菌与真菌群落进行了表征。