GSE47313: LSD1_Q3430.OR Head Nuclei.Solexa

Summary: modENCODE_submission_5574 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using the Illumina NGS platform. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Overall Design: EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Developmental Stage: Mixed Adult; Genotype: wild type; EXPERIMENTAL FACTORS: Strain Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); tissue (organism part) ; Antibody LSD1 Q3430 (target is LSD1); Developmental Stage Mixed Adult

摘要：modENCODE提交数据集5574
本数据集提交自Gary Karpen主导的modENCODE项目。如需查看modENCODE项目完整列表，请访问http://www.genome.gov/26524648。
项目目标：本项目旨在定位黑腹果蝇（Drosophila melanogaster）基因组内125种染色体蛋白的分布位点。本次研究的蛋白参与DNA复制、基因表达、基因沉默及遗传等核心染色体生物学功能。本研究将依托Illumina二代测序（NGS）平台开展染色质免疫沉淀（Chromatin ImmunoPrecipitation, ChIP）实验，初期将使用三种细胞系与两个胚胎时期的染色质进行蛋白定位检测，后续将针对部分蛋白将分析范围拓展至另外四种动物组织/发育阶段。
关于数据使用条款与规范，请参阅http://www.genome.gov/27528022及http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。
整体设计：
实验类型：ChIP-seq。
生物样本来源：品系：Oregon-R（官方命名：Oregon-R-modENCODE，基因型：野生型）；发育阶段：混合成虫；基因型：野生型；
实验因素：品系Oregon-R（官方命名：Oregon-R-modENCODE，基因型：野生型）；组织（生物体部位）；抗体：LSD1 Q3430（靶标为LSD1）；发育阶段：混合成虫