Scintigraphic study of the splenic circulation in rats

Objective: To evaluate the splenic parenchymal blood distribution through scintigraphic study.Methods: Thirty Wistar rats were randomly divided into six groups (n = 5). Group 1 (spleen - 30 minutes) and Group 2 (spleen - 90 minutes) underwent laparotomy with direction of blood flow to the spleen by ligature of the aorta near the iliac bifurcation and splanchnic vessels, keeping blood flow only in the splenic artery; Group 3 (spleen and stomach - 30 minutes) and Group 4 (spleen and stomach - 90 minutes) underwent laparotomy with direction of blood flow to the spleen and stomach by ligature of the aorta near the iliac bifurcation and splanchnic vessels, maintaining the flow through the splenic, gastric and splenogastric vessels; Group 5 (control - 30 minutes) and Group 6 (control - 90 minutes) underwent laparotomy and ligation of the aorta near the iliac bifurcation, keeping the flow to the abdominal organs. After arterial ligation, the animals received an injection of 0.2 ml of sodium pertechnetate in the aorta. Scintigraphic images were taken and the animals had their spleens removed for radioactivity counting with an automatic counter device.Results: There was no difference in the amounts of radiation from the spleen between groups, indicating retention of the radioisotope by the spleen, even after the period of 90 minutes.Conclusion: The blood flow through the spleen is not continuous. The blood diffuses through the splenic parenchyma and its venous drainage is slow, not following a predictable sequence.

研究目的：本研究旨在通过闪烁显像（scintigraphic study）技术评估脾脏实质的血液分布情况。
研究方法：选取30只Wistar大鼠，随机分为6组，每组5只。第1组（脾脏-30分钟组）与第2组（脾脏-90分钟组）实施剖腹手术，通过结扎髂分叉处的主动脉及内脏血管，使血流仅经脾动脉流向脾脏；第3组（脾与胃-30分钟组）与第4组（脾与胃-90分钟组）实施剖腹手术，结扎髂分叉处的主动脉及内脏血管，保留脾动脉、胃动脉及脾胃血管的血流，使血流同时流向脾脏与胃部；第5组（对照-30分钟组）与第6组（对照-90分钟组）实施剖腹手术并结扎髂分叉处的主动脉，保留流向腹部脏器的血流。动脉结扎完成后，向大鼠主动脉注射0.2ml高锝酸钠（sodium pertechnetate）溶液。随后采集闪烁显像图像，并摘除大鼠脾脏，使用自动计数设备进行放射性活度计数。
研究结果：各组脾脏的放射性剂量无显著差异，表明即使在90分钟后，放射性同位素仍滞留在脾脏内。
研究结论：脾脏的血流并非持续灌注；血液可弥散至脾脏实质，且其静脉引流速度缓慢，未遵循可预测的血流序列。