17-DMAG treatment in primary CLL B cells. Homo sapiens

We used microarrays to analyze gene expression following treatment of leukemic B cells with the Hsp90 inhibitor 17-DMAG. Gene expression profiling reveals the role of SOCS3 in cytokine signaling in CLL Overall design: Primary cells from CLL patients were isolated and treated in vitro with 17-DMAG. Cells were collected for viability, gene expression analysis and cell signaling and migration assays.

本研究采用微阵列（microarray）技术，分析经热休克蛋白90（Hsp90）抑制剂17-DMAG处理的白血病B细胞的基因表达情况。基因表达谱分析揭示了SOCS3（Suppressor of Cytokine Signaling 3）在慢性淋巴细胞白血病（Chronic Lymphocytic Leukemia, CLL）的细胞因子信号通路中的作用。实验设计概述：从CLL患者体内分离原代细胞，体外经17-DMAG处理后，收集细胞用于细胞存活率检测、基因表达分析以及细胞信号转导与迁移实验。