Comprehensive single-cell mRNA profiling reveals a detailed roadmap for pancreatic endocrinogenesis

This dataset consists of single-cell RNA-seq (10X) data from 4 embryonic stages (E12.5-15.5) of pancreatic epithelial cells from Neurogenin3 (Ngn3)-Venus fusion (NVF) homozygous mice. Endocrine progenitor cells (NVF+) were enriched by FACS cell sorting. 4 single cell RNA seq experiments were performed using 10X Genomics technology to generate single cell transcriptomic profiles of embryonic pancreatic epithelial cells from a Neurogenin3 (Ngn3)-Venus fusion (NVF) reporter mouse line. Cells were sorted and NVF+ and NVF- cells mixed (50:50) to enrich for endocrine Ngn3+ progenitors. Cells were sampled from pancreata of E12.5,E13.5,E14.5 and E15.5 embryos.

本数据集包含来自4个胚胎发育阶段（E12.5至E15.5）的神经元素3（Neurogenin3, Ngn3）-Venus融合（NVF）纯合小鼠的胰腺上皮细胞的单细胞RNA测序（10X）数据。研究通过荧光激活细胞分选（FACS）富集内分泌祖细胞（NVF+），并采用10X Genomics技术开展4次单细胞RNA测序实验，以获取该神经元素3（Ngn3）-Venus融合（NVF）报告基因小鼠品系的胚胎胰腺上皮细胞的单细胞转录组图谱。具体实验流程为：对细胞进行分选后，将NVF+与NVF-细胞按50:50的比例混合，以富集内分泌型Ngn3+祖细胞；细胞样本采集自E12.5、E13.5、E14.5及E15.5胚胎的胰腺组织。