Exome sequencing of human ex vivo ovarian cancer models with matched stromal cells

Ascites or solid tumour from patients with ovarian cancer was collected and grown in culture as ex vivo models. Each sample has a mixture of tumour and stromal cells which were separated into individual cultures. Therefore each patient has tumour and stromal cultures originating from the same tissue collection. Variant calling (exome-seq) analysis was performed on these matched models to establish tumour specific mutations. Stromal cells were used to rule out germline mutations.

本数据集采集卵巢癌患者的腹水或实体瘤组织，将其体外培养为离体（ex vivo）模型。每份样本均包含肿瘤细胞与基质细胞的混合群体，随后将两类细胞分离并分别建立独立培养体系。因此每位患者的肿瘤细胞与基质细胞培养体系均源自同一批组织样本。研究人员对上述配对模型开展基于外显子组测序（exome-seq）的变异识别（variant calling）分析，以鉴定肿瘤特异性突变；同时利用基质细胞培养体系排除生殖系突变（germline mutations）的干扰。