The landscape of H3K27me2 in mice zygotes

H3K27 di-methylation and H3K27 trimethylation are marks of facultative heterochromatin which maintains transcriptional repression established during early development in many eukaryotes. However, the mechanism underlying establishment and regulation of epigenetic asymmetry in the zygote remains obscure. Here we report the distribution pattern of H3K27me2 in Ezh2m+/p+ and Ezh2m-/p+ mice zygotes using the approach of ULI-NChIP. Overall design: ULI-NChIP profiling of H3K27me2 in Ezh2m+/p+ and Ezh2m-/p+ mice zygotes.

H3K27二甲基化（H3K27 di-methylation）与H3K27三甲基化（H3K27 trimethylation）是兼性异染色质（facultative heterochromatin）的标志性修饰，可维持多数真核生物早期发育过程中建立的转录抑制状态。然而，受精卵（zygote）中表观遗传不对称（epigenetic asymmetry）的建立与调控机制仍不甚明晰。本研究借助ULI-NChIP技术，解析了Ezh2m+/p+与Ezh2m-/p+小鼠受精卵中H3K27me2的分布模式。实验整体设计：采用ULI-NChIP技术对Ezh2m+/p+与Ezh2m-/p+小鼠受精卵中的H3K27me2进行谱分析。