Summary of RegR binding studies with promoter regions of anoxically induced, RegR-controlled B. japonicum genes.

aAll listed genes are differentially expressed (FCregR mutant both grown under the anoxic, denitrifying conditions as described in this work.bGene name as indicated in the EMBL-EBI database.cProtein description according to Kaneko and coworkers, 2002 [67].dGenomic region included in the PCR fragment used for EMSAs. Coordinates refer to the first nucleotide position relative to the annotated translation start site of the genes listed in column 1.eIndicates qualitatively whether (+) or not (−) RegR binding was observed to the DNA fragments specified in column 4.fPosition of the 5′-end nucleotide of the putative RegR box (column 7) relative to the annotated translational start site of the associated gene.gSequence of the putative RegR binding sites. Conserved nucleotides are highlighted.

a. 所列全部基因均为差异表达基因（FCregR突变体均按照本文所述的缺氧、反硝化条件进行培养）。b. 基因名称以EMBL-EBI数据库中的标注为准。c. 蛋白质注释参考Kaneko及其同事2002年发表的文献[67]。d. 本研究用于电泳迁移率变动分析（EMSAs）的PCR片段所覆盖的基因组区域，其坐标相对于第1列所列基因的注释翻译起始位点的第一个核苷酸位置。e. 定性标注RegR与第4列指定DNA片段的结合情况：(+)表示观测到RegR结合，(-)表示未观测到RegR结合。f. 推定RegR结合框（第7列）的5'端核苷酸位置，相对于对应基因的注释翻译起始位点。g. 推定RegR结合位点的序列，保守核苷酸已高亮标注。