NiV infection transcriptomics profile on HEK29T cells

To delineate cellular processes involved in NiV infection, we have employed whole genome microarray expression profiling as a discovery platform to identify the upregulated and downregulated genes. Total RNA was collected from infected cells and uninfected controls. Cy3 labeled cRNA is used for hybridization platform Agilent Human 4 x 44K gene expression array. Time-course based infections were performed at moi 1. 4h, 8h, 12h, 16h infections were compared to uninfected HEK293T cell transcriptomics. Five replicates were performed for each condition, and labeled as A through E.

为厘清尼帕病毒（Nipah Virus, NiV）感染相关的细胞过程，本研究采用全基因组微阵列表达谱作为发现平台，以筛选感染过程中上调与下调的基因。实验从感染细胞与未感染对照细胞中提取总RNA，将Cy3标记的互补RNA（complementary RNA, cRNA）用于安捷伦（Agilent）人类4×44K基因表达芯片的杂交实验。本研究以感染复数（multiplicity of infection, MOI）=1开展时间梯度感染实验，分别在感染后4h、8h、12h、16h收取样本，并与未感染HEK293T细胞的转录组进行比对。每个实验条件均设置5次生物学重复，编号为A至E。