Patterns of insecticide resistance and knock down resistance (kdr) in malaria vectors An. arabiensis, An. coluzzii and An. gambiae from sympatric areas in Senegal

Sibling species identification and kdr mutation detection were determined using polymerase chain reaction on mosquitoes collected using pyrethrum sprays collection in 20 sites belonging to two transects with differential insecticide selection pressure. The WHO tube test was used to determine phenotypic resistance of An. gambiae s.l. to DDT, deltamethrin, lambdacyholothrin, permethrin, bendiocarb and malathion in four representative sites. (MapVEu VBP0000156)

本研究采用聚合酶链式反应（polymerase chain reaction），对在两条具有差异化杀虫剂选择压力的样带内的20个采集点中，通过拟除虫菊酯喷雾法捕获的蚊虫，开展姊妹种鉴定与击倒抗性（kdr）突变检测。在四个代表性采集点中，采用世卫组织管碟测试法，检测冈比亚按蚊复合组（An. gambiae s.l.）对滴滴涕（DDT）、溴氰菊酯、氯氟氰菊酯、氯菊酯、残杀威以及马拉硫磷的表型抗性。（数据集编号：MapVEu VBP0000156）