Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas

Abstract The study aimed to evaluate the antimicrobial activity, antioxidant, toxicity and phytochemical screening of the Red Propolis Alagoas. Antimicrobial activity was evaluated by disk diffusion method. Determination of antioxidant activity was performed using the DPPH assay (1.1-diphenyl-2-picrylhydrazyl), FTC (ferric thiocyanate) and determination of phenolic compounds by Follin method. Toxicity was performed by the method of Artemia salina and cytotoxicity by MTT method. The phytochemical screening for the detection of allelochemicals was performed. The ethanol extract of propolis of Alagoas showed significant results for antimicrobial activity, and inhibitory activity for Staphylococcus aureus and Candida krusei. The antioxidant activity of the FTC method was 80% to 108.3% hydrogen peroxide kidnapping, the DPPH method showed an EC50 3.97 mg/mL, the content of total phenolic compounds was determined by calibration curve gallic acid, resulting from 0.0005 mg/100 g of gallic acid equivalent. The extract was non-toxic by A. salina method. The propolis extract showed high activity with a higher percentage than 75% inhibition of tumor cells OVCAR-8, SF-295 and HCT116. Chemical constituents were observed as flavonones, xanthones, flavonols, and Chalcones Auronas, Catechins and leucoanthocyanidins. It is concluded that the extract can be tested is considered a potential source of bioactive metabolites.

摘要：本研究旨在评估阿拉戈斯红蜂胶（Red Propolis Alagoas）的抗菌活性、抗氧化活性与毒性，并开展其植物化学筛选。抗菌活性采用纸片扩散法检测；抗氧化活性分别通过1,1-二苯基-2-苦基肼（DPPH）法、硫氰酸铁（FTC）法测定；酚类物质含量采用福林（Follin）法进行定量。毒性检测采用卤虫（Artemia salina）法，细胞毒性则采用MTT法完成。本研究同时完成了化感物质的植物化学筛选。阿拉戈斯红蜂胶的乙醇提取物展现出显著的抗菌活性，对金黄色葡萄球菌（Staphylococcus aureus）与克鲁斯假丝酵母（Candida krusei）具有抑制作用。经FTC法测定，其过氧化氢清除率为80%~108.3%；DPPH法测得其半数有效浓度（EC50）为3.97 mg/mL；以没食子酸标准曲线标定总酚类物质含量，结果为每100克样品对应0.0005 mg没食子酸当量。经卤虫法检测，该提取物无毒性。该蜂胶提取物对卵巢癌细胞系OVCAR-8、SF-295及结肠癌细胞系HCT116的肿瘤细胞抑制率均高于75%，活性优异。其所含化学成分包括黄烷酮类、呫吨酮类、黄酮醇类、查尔酮类与奥诺酮类、儿茶素类以及无色花色素类。综上，该提取物可作为后续实验候选对象，是极具潜力的生物活性代谢物来源。