Metabolomic changes in lactating multiparous naturally MAP-infected Holstein-Friesian dairy cows highlight changes in mitochondrial energy pathways

The study used biobanked milk samples which were collected 73.4 ± 3.79 (early lactation) and 143 ± 3.79 (mean ± SE) (mid-lactation) days post-calving from 5 MAP-infected and 5 control multiparous cows. The milk metabolome was assessed using flow infusion electrospray high-resolution mass spectrometry (FIE-HRMS) for high throughput, sensitive, non-targeted metabolite fingerprinting.

本研究使用生物保藏的牛乳样本，该类样本采集自5头感染副结核分枝杆菌（Mycobacterium avium subsp. paratuberculosis，MAP）的经产母牛与5头对照经产母牛，采样时间为产犊后73.4±3.79天（泌乳早期），以及产犊后143±3.79天（均值±标准误，泌乳中期）。本研究采用流动注射电喷雾高分辨质谱法（flow infusion electrospray high-resolution mass spectrometry，FIE-HRMS）对牛乳代谢组进行检测，以实现高通量、高灵敏度的非靶向代谢物指纹分析。