RNA-seq of DHA-treated primary mantle cells from the razor clam Sinonovacula constricta

To elucidate the transcriptomic response of DHA-induced lipid accumulation in marine mollusks, we used the razor clam Sinonovacula constricta as a model. Primary mantle cells were treated with 50 uM DHA for 6 hours, followed by RNA sequencing of the collected cell samples to identify key differentially expressed genes and regulatory pathways.

为阐明二十二碳六烯酸（Docosahexaenoic Acid，DHA）诱导海洋软体动物脂质蓄积的转录组响应，本研究以缢蛏（Sinonovacula constricta）作为实验模型。我们将原代外套膜细胞以50 μM DHA处理6小时，随后对收集的细胞样本进行RNA测序，以鉴定关键差异表达基因与调控通路。