ChEC-Seq: a robust method to identify protein-DNA interactions genome-wide

Here we show that the ChEC-Seq technique is able to differentiate the binding specificities of Esa1 and Gcn5 two chromatin-binding factors displaying widespread genome-wide associations. We also show that the ChEC-Seq technique reveals strong binding of the transcription factor Sfp1 at Ribi gene promoters. Furthermore, our data provide the first evidence that a specific DNA motif previously identified by ChEC-Seq (Albert 2019 PMID: 30804227) is in fact an in vivo binding site for Sfp1. ChEC-Seq of Esa1 and Gcn5 in S.cerevisiae cells. ChEC-Seq of Sfp1 in wild type and pYPL245-mutant S.cerevisiae cells.

本研究证实，ChEC-Seq技术可区分Esa1与Gcn5两种染色质结合因子的结合特异性，二者在全基因组范围内存在广泛的结合关联。本研究同时证明，ChEC-Seq技术可揭示转录因子Sfp1在Ribi基因启动子区域的强结合信号。此外，本研究数据首次提供直接证据，证实此前由ChEC-Seq（Albert等，2019，PMID: 30804227）所鉴定的特定DNA基序，实则为Sfp1的体内结合位点。本数据集包含酿酒酵母（S.cerevisiae）细胞中Esa1与Gcn5的ChEC-Seq测序数据，以及野生型与pYPL245突变型酿酒酵母细胞中Sfp1的ChEC-Seq测序数据。