Tracheal aspirate single cell RNA sequencing from patients with ARDS [scRNA-seq]

Trachel aspirates samples were collected from an observational cohort of mechanically ventilated patients to compare respiratory tract gene expression in previous-descried ARDS molecular phenotypes. Patient charts were reviewed by two physicians and patients with ARDS were identified using the Berlin Definition. ARDS molecular phenotypes were defined using a previously-described 3-variable classifier (Sinha 2020) Tracheal aspirates were collected from mechnically ventilated patients admitted to the intensive care units. ***Please note that the UCSF IRB has restricted sharing the raw data because some of the samples were collected under a waiver of consent and could in theory be identifiable. Therefore, raw data is not provided***

本研究从入住重症监护病房的机械通气患者的观察性队列中采集气管抽吸物（Tracheal aspirates）样本，旨在对比既往报道的急性呼吸窘迫综合征（ARDS）分子表型对应的呼吸道基因表达特征。由两名医师审阅患者病历，并采用柏林定义（Berlin Definition）确诊急性呼吸窘迫综合征患者。急性呼吸窘迫综合征的分子表型通过既往报道的三变量分类器（Sinha 2020）进行界定。***请注意：加州大学旧金山分校伦理审查委员会（UCSF IRB）已限制原始数据共享，因部分样本是在豁免知情同意的前提下采集的，理论上存在可识别性风险。因此本数据集不提供原始数据。***