MYC2 and MYC3 orchestrate pith lignification to defend Nicotiana attenuata stems against a stem-boring weevil

To investigate how NaMYC2 and NaMYC3 contribute to stem-boring herbivore-induced lignification, we analyzed global transcriptional changes in T. mucorea-infested pith tissues of WT, Namyc2, Namyc3, and Namyc2/3 using RNA-seq. Gene expression in control (C) and T. mucorea-infested (D) pith of Nicotiana attenuata wildtype (WT), Namyc2 (myc2), Namyc3 (myc3), and Namyc2/3 (mycD). Three biological replicates were performed with both wild type and Namyc mutants.

为探究NaMYC2与NaMYC3如何介导蛀茎植食性昆虫诱导的木质化过程，本研究通过RNA测序（RNA-seq）分析了野生型（WT）、Namyc2、Namyc3以及Namyc2/3植株的髓组织在被T. mucorea侵染后的全局转录组变化。本研究检测了渐狭烟草（Nicotiana attenuata）野生型（WT）、Namyc2（myc2）、Namyc3（myc3）以及Namyc2/3（mycD）的对照组（C）与T. mucorea侵染组（D）的髓组织基因表达水平。野生型与所有Namyc突变体均设置三次生物学重复。