Prevalence of culicine salivary antibodies in non-human primates living in national parks in Thailand

Macaques are widely distributed in Thailand with remarkable adaptation to living close to humans in residential, religious sites, markets, and tourist areas. They play an essential role in the persistence of pathogens in the environment. As reservoir hosts, they are exposed to hematophagous vectors that secrete saliva, a cocktail of bioactive molecules including antigenic components stimulating host antibody production. Subsequent to the detection of mosquito-borne pathogens in macaques living in national parks, we aimed to determine the seroprevalence of antibodies to crude salivary gland extracts (SGE) from culicine mosquitoes (Aedes aegypti [Linnaeus, 1762], Ae. albopictus [Skuse, 1895], and Culex quinquefasciatus [Say, 1823]) and compare individual titers between macaque species/national parks (33, Macaca arctoides [I.Geoffroy Saint-Hilaire, 1831] [Primates: Cercopithecidae] from Kaeng Krachan, 23 M. leonina leonina [Blyth, 1863] [Primates: Cercopithecidae] from Khao Yai, and 4 M. f..., All data sets of antibody titers of macaque bloods, mosquito salivary grand extraction protein profiles, and PRNT titers of virus detections were recored in Microsolf excel spreed sheet solfware, USA., , # Dataset-Med Vet Entomo

[https://doi.org/10.5061/dryad.tht76hf7z](https://doi.org/10.5061/dryad.tht76hf7z)

## Description of the data and file structure

Figure1 file: data set Map of serum samples collected from macaques in Thailand national parks from 2018 to 2019.

Fig2 file: Immunoglobulin G SGE (salivary gland extract) antibody endpoint titers (log2) to three mosquito species: *Aedes aegypti*(circle), *Ae. albopictus*(square), and *Culex quinquefasciatus*(triangle) from national park-dwelling macaques determined by ELISA. Serum samples were collected from *M. leonina, M. arctoides,* and *M. fascicularis* from Khao Yai (KY) National Park in Nakhon Ratchasima, Kaeng Krachan (KK) National Park in Prachuap Khiri Khan, and Mu Ko Ranong (RN) National Park in Ranong, respectively.

Fig3 file: Salivary gland extract antibody titers (log2) against *Aedes aegypti*(A) and *Ae. albopictus*(B) between dengue virus/DENV- (A) and Zika virus/ZIKV- (B) positive (+ve) and -negative (-ve) stump-ta...,

猕猴在泰国分布广泛，且能极佳适应与人类共处的人居、宗教场所、集市及旅游景区。它们在病原体在环境中的持续存续过程中发挥关键作用。作为病原体储存宿主，猕猴会接触吸血媒介节肢动物，这类媒介会分泌含有多种生物活性分子的唾液，其中包含可刺激宿主产生抗体的抗原成分。在针对国家公园内猕猴体内蚊媒病原体的检测工作完成后，本研究旨在明确库蚊属蚊虫（埃及伊蚊*Aedes aegypti* [Linnaeus, 1762]、白纹伊蚊*Ae. albopictus* [Skuse, 1895]以及致倦库蚊*Culex quinquefasciatus* [Say, 1823]）的粗唾液腺提取物（Salivary Gland Extract, SGE）抗体的血清阳性率，并比较不同猕猴物种/国家公园间的个体抗体滴度：共33只熊猴（*Macaca arctoides* [I.Geoffroy Saint-Hilaire, 1831] [灵长目: 猴科]）采集自岗卡章国家公园，23只北部豚尾猴（*M. leonina leonina* [Blyth, 1863] [灵长目: 猴科]）采集自考艾国家公园，以及4只食蟹猴（原文缩写为*M. f.*）。所有猕猴血清抗体滴度、蚊唾液腺提取物蛋白谱以及病毒检测的空斑减少中和试验（Plaque Reduction Neutralization Test, PRNT）滴度数据，均记录于美国微软（Microsoft）Excel电子表格软件中。# Dataset-Med Vet Entomo
https://doi.org/10.5061/dryad.tht76hf7z

## 数据与文件结构说明
图1文件：2018-2019年于泰国国家公园内采集的猕猴血清样本分布图。
图2文件：采用酶联免疫吸附试验（Enzyme-Linked Immunosorbent Assay, ELISA）测定的、来自国家公园栖息猕猴的三种蚊子（埃及伊蚊[圆形标记]、白纹伊蚊[方形标记]、致倦库蚊[三角形标记]）的免疫球蛋白G（IgG）唾液腺提取物（SGE）抗体终点滴度（log₂转换值）。血清样本分别采集自：那空叻差是玛府考艾国家公园的北部豚尾猴（*M. leonina*）、巴蜀府岗卡章国家公园的熊猴（*M. arctoides*）以及拉廊府拉廊群岛国家公园的食蟹猴（*M. fascicularis*）。
图3文件：登革病毒/DENV阳性（+ve）与阴性（-ve）样本（A）、寨卡病毒/ZIKV阳性与阴性样本（B）之间，针对埃及伊蚊（A）与白纹伊蚊（B）的唾液腺提取物抗体滴度（log₂转换值）对比。