Cut&Run of Aldh18a1 using 46C mouse ES cells

Using a proteomic approach we identified Aldh18a1 as a protein co-localized with RNA polymerase II. We then carried out Aldh18a1 Cut&Run assays to confirm its co-localization with RNA polymerase II (E-MTAB-11510). As a result, we observed Aldh18a1 is co-localized with RNA polymerase II primarily on transcription start sites.

本研究采用蛋白质组学方法，将Aldh18a1鉴定为一种与RNA聚合酶II（RNA polymerase II）共定位的蛋白质。随后，我们针对Aldh18a1开展了Cut&Run实验，以验证其与RNA聚合酶II的共定位关系（数据集登录号：E-MTAB-11510）。结果表明，Aldh18a1主要在转录起始位点处与RNA聚合酶II发生共定位。