Establishment of bovine trophoblast stem cells [WGBS]

Here we report that a chemical cocktail (LCDM: hLIF, CHIR99021, DiM and MiH) previously reported for extended potential pluripotent stem cells enables the de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs exhibit transcriptomic and epigenetic features characteristic of trophectoderm cells from bovine embryos and retain developmental potency to differentiate into functional trophoblasts in vitro and in vivo Whole genome bisulfite sequencing analysis was used to determined genome-scale DNA methylation patterns of bTSC, D7_TE, and D14_TE.

本研究报道，一种此前被用于培养扩展潜能多能干细胞的化学组合（LCDM：hLIF、CHIR99021、DiM与MiH），可实现牛滋养层干细胞（trophoblast stem cells, TSCs）的从头建系与长期体外培养。该细胞系具备牛胚胎滋养外胚层（trophectoderm）细胞特有的转录组与表观遗传特征，并保留了在体外（in vitro）与体内（in vivo）分化为功能性滋养层细胞的发育潜能。本研究采用全基因组亚硫酸氢盐测序（whole genome bisulfite sequencing）分析，对bTSC、第7天滋养外胚层（D7_TE）及第14天滋养外胚层（D14_TE）的全基因组DNA甲基化模式进行了测定。