Transcriptional characterization of PD-1 positive and negative Foxp3 negative CD4 positive T cells from liver of 5- and 10-day-old Aire-deficient C57BL/6 mice

The first T cells to arrive in the liver were mostly T regulatory (Treg) cells and metabolically active, highly proliferative T conventional (Tconv) cells. The Tconv cells had unusually high expression of PD-1 and the IL-33 receptor, ST2. As these PD-1+ Tconv cells accumulated in the tissue, they gradually lost their expression of ST2, ceased to proliferate and acquired an anergic phenotype. Overall design: Gene expression profiles of flow cytometry sorted DAPI negative CD45 positive TCRb positive CD4 positive Foxp3 negative cells from liver of 5- and 10-day-old B6.Aire-KO mice

首批抵达肝脏的T细胞主要为调节性T（T regulatory, Treg）细胞，以及代谢活跃、高增殖性的常规T（T conventional, Tconv）细胞。此类Tconv细胞会异常高表达程序性死亡受体1（PD-1）与IL-33受体ST2。随着这些PD-1阳性的Tconv细胞在肝组织中不断蓄积，它们会逐步丧失ST2的表达、停止增殖，并获得无反应性表型。实验整体设计：对5日龄和10日龄B6.Aire基因敲除小鼠的肝脏中，经流式细胞术分选的DAPI阴性、CD45阳性、TCRβ阳性、CD4阳性、Foxp3阴性的细胞开展基因表达谱分析。