Single cell RNA-seq of adult human neural retina. Single cell RNA-seq of adult human neural retina

To comprehensively profile cell types in the human retina, we performed single cell RNA-sequencing on 20,009 cells obtained post-mortem from three donors and compiled a reference transcriptome atlas. Using unsupervised clustering analysis, we identified 18 transcriptionally distinct clusters representing all known retinal cells: rod photoreceptors, cone photoreceptors, Müller glia cells, bipolar cells, amacrine cells, retinal ganglion cells, horizontal cells, retinal astrocytes and microglia.

为全面解析人类视网膜中的细胞类群，我们对3名已故捐献者死后获取的20009个细胞开展了单细胞RNA测序（single cell RNA-sequencing），并构建了参考转录组图谱。借助无监督聚类分析（unsupervised clustering analysis），我们鉴定出18个转录特征各异的细胞簇，涵盖了所有已知的视网膜细胞类型：视杆感光细胞、视锥感光细胞、米勒胶质细胞（Müller glia cells）、双极细胞、无长突细胞、视网膜神经节细胞、水平细胞、视网膜星形胶质细胞以及小胶质细胞。