E. coli Sacculi +A22 (MG1655)

Tilt Series Date: 2015-04-10 Data Taken By: Matt Swulius Species / Specimen: Escherichia coli Strain: MG1655 sacculi Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. Microscope: Caltech Polara Acquisition Software: UCSFTomo Upload Method: pipeline Processing Software Used: raptor Sample Preparation: Cells were treated for one hour with 10ug/ml A22 until they were rounded and lemon-shaped prior to sacculus purification.

倾斜序列采集日期：2015-04-10
数据采集人员：Matt Swulius
物种/样品：大肠杆菌（Escherichia coli），菌株：MG1655 肽聚糖囊（sacculi）
倾斜序列采集参数：单轴倾斜模式，倾斜范围为(-60.0°, 60.0°)，步长1°，采用恒定角度增量，电子剂量为150.0 eV/Ų，散焦值为-6.0 μm，放大倍数为22500×
显微镜：Caltech Polara透射电子显微镜
采集软件：UCSFTomo
上传方式：流水线上传
所用数据处理软件：raptor
样品制备：在开展肽聚糖囊纯化前，将细胞以10μg/ml浓度的A22处理1小时，直至细胞变为圆形及柠檬形。