Transcriptional dysregulation impairs efficacy of neural stem cell activation during aging [RNA-seq]

To identify age-dependent genes of NSCs, we applied bulk RNA sequencing to profile the transcriptomic changes between the young and aged NSCs that were isolated from the brain SVZ. Primary NSCs were isolated from the subventricular zone of the lateral ventricles (SVZ) in the brains of the young and aged mice, respectively. The isolated NSCs were maintained in the initial proliferation medium and used in total RNA extraction and bulk RNAseq library generation.为鉴定神经干细胞（Neural Stem Cells, NSCs）的年龄依赖性基因，我们采用批量RNA测序（bulk RNA sequencing）对从大脑侧脑室下区（Subventricular Zone, SVZ）分离得到的年轻与老年神经干细胞的转录组变化进行分析。实验分别从年轻及老年小鼠大脑的侧脑室下区（SVZ）分离原代神经干细胞。将分离获得的神经干细胞置于初代增殖培养基中培养，随后用于总RNA提取与批量RNA测序文库构建。