Whole-Genome Analysis of SSB Larvae Infestation Regulated Rice Genes

Rice, the world’s most important food crop, is attacked by multiple herbivores and pathogens.the rice striped stem borer (SSB) Chilo suppressalis is one of another most important rice insect pests. Here, we use Affymetrix Whole-Genome rice arrays to detect SSB infestation responsive genes. RNA samples from five damaged stems of SSB-challenged 24 h rice plants or five stems from unchallenged plants (for control samples) were used to array analysis. Two replicate biological experiments of SSB treatment rice arrays and one control samples array were peformed.

水稻作为全球最重要的粮食作物，会受到多种植食性昆虫与病原物的侵袭。稻条纹螟（二化螟，*Chilo suppressalis*，SSB）便是水稻最主要的害虫之一。本研究采用Affymetrix水稻全基因组芯片（Affymetrix Whole-Genome rice arrays），以检测受二化螟侵染后的响应基因。我们分别采集了受二化螟侵染24小时的水稻植株的5根受损茎秆，以及未受侵染的水稻植株的5根茎秆（作为对照样本），提取其RNA样品用于芯片分析。本研究共开展了2组二化螟处理组水稻芯片的生物学重复实验，以及1组对照样本的芯片实验。