Photosynthetic primary production of microplankton combined with different size fractions in sea water from the euphotic zone of the Aegean Sea at Station SEPT-1997-GN36199704605MSB01

Primary production was measured with the in situ 14C method of Steeman-Nielsen (Steemann-Nielsen, E. 1952. The use of radio-active carbon 14C for measuring organic production in the sea. Journal du Conseil International pour l'Exploration de la Mer. 18:3, 117-140). Samples were dispensed in 250 ml transparent polycarbonate bottles (three light and one dark for each depth); and each one was inoculated with 5 µCi 14-C-NaHCO3 and incubated at the collection depths for 2 hours. Fractionation was carried out by filtration through 0.2 µm, 1.2 µm and 3.0 µm polycarbonate Millipore filters. Filters were placed in scintillation vials, acidified with 0.5 N HCl and counted in a liquid scintillation counter.

本研究采用Steeman-Nielsen原位14C法（Steemann-Nielsen, E. 1952. 放射性碳14C在海洋有机生产量测定中的应用. 《国际海洋考察理事会期刊》, 18卷3期, 117-140）对初级生产量进行测定。实验样品分装于250 mL透明聚碳酸酯（polycarbonate）瓶中，每一层采样深度设置3个光照培养瓶与1个暗培养瓶；每瓶接种5微居里的14C标记碳酸氢钠（14C-NaHCO3），并在原采样深度下培养2小时。随后通过0.2 µm、1.2 µm及3.0 µm孔径的聚碳酸酯密理博（Millipore）滤膜进行分级过滤分离。将滤膜转移至闪烁瓶中，用0.5当量盐酸（0.5 N HCl）酸化后，使用液体闪烁计数器完成放射性活度计数。