Adrenergic signaling stimulates body-wide stem cell activation for limb regeneration (2N RNA-Seq). Adrenergic signaling stimulates body-wide stem cell activation for limb regeneration (2N RNA-Seq)

In axolotls, stem cells are systemically prompted to re-enter the cell cycle upon amputation. We sought to identify how the cells surrounding the systemically activated cells respond to an amputation. Overall design: We dissociated homeostatic or systemically-activated limbs at 14 days post-amputation, and sorted them by their DNA content using FACS. We then performed single-cell RNA sequencing of the dividing, 2C population using 10X genomics.

在美西钝口螈（axolotl）中，干细胞会在断肢后被系统性诱导重新进入细胞周期。本研究旨在阐明系统性激活的细胞周围的细胞对断肢刺激的响应机制。实验设计概述：我们分别解离了稳态状态下的肢体组织，以及断肢后14天的系统性激活肢体组织；随后通过荧光激活细胞分选术（Fluorescence-Activated Cell Sorting, FACS）基于细胞的DNA含量完成细胞分选。最后，使用10× Genomics平台对分裂中的2C细胞群体开展单细胞RNA测序。