Sequence alignment fasta files of 634 loci from 5 Heliconius species

This is a compressed directory of 634 anonymous loci obtained from RNA-Seq data and aligned for phylogenetic analysis. The data come from 5 Heliconius species: Heliconius melpomene, Heliconius erato, Heliconius sara, Heliconius doris, and Heliconius charithonia. Briefly, we performed Illumina RNA sequencing followed by de novo transcriptome assembly. BLAST was used to determine orthology across species and the selected loci were aligned using Clustal W. This file contains the final 634 loci used to reconstruct the Heliconius phylogeny. The file is a zipped directory containing each locus as a separate aligned fasta file. File names include the original (arbitrary) locus number and the length of the locus, but loci themselves are anonymous (i.e., are not annotated/named). Each file contains 5 sequences labelled as so: char (Heliconius charithonia), dori (Heliconius doris), erat (Heliconius erato), melp (Heliconius melpomene), sara (Heliconius sara). See publication Methods section for a more detailed description of methodology.

本数据集为经压缩的目录文件，包含634个匿名基因座（loci，单数形式为locus），均源自RNA测序（RNA-Seq）数据，经多序列比对后用于系统发育分析。该数据来源于5种袖蝶属（Heliconius）昆虫，分别为诗袖蝶（Heliconius melpomene）、红带袖蝶（Heliconius erato）、萨拉袖蝶（Heliconius sara）、桃金娘袖蝶（Heliconius doris）以及白裙袖蝶（Heliconius charithonia）。简言之，本研究先通过Illumina平台完成RNA测序，随后进行从头转录组组装（de novo transcriptome assembly）；利用BLAST鉴定跨物种的直系同源基因，并通过Clustal W对筛选得到的基因座进行多序列比对。本文件包含最终用于重建袖蝶属系统发育树的全部634个基因座，其为压缩目录，每个基因座对应一个独立的比对后fasta格式文件。文件名包含原始（随机分配的）基因座编号及该基因座的序列长度，但基因座本身未做注释或命名，处于匿名状态。每个文件内包含5条序列，标注缩写对应物种如下：char为白裙袖蝶（Heliconius charithonia）、dori为桃金娘袖蝶（Heliconius doris）、erat为红带袖蝶（Heliconius erato）、melp为诗袖蝶（Heliconius melpomene）、sara为萨拉袖蝶（Heliconius sara）。详细的实验方法请参阅相关发表论文的方法章节。