Analysis of Nkx2-1-regulated gene expression in A549 lung carcinoma cells. Homo sapiens

A transcription factor Nkx2-1 (also known as TTF-1) regulates the expression of different sets of genes. Gene expression analysis was performed using mRNAs from Nkx2-1-induced A549 cells compared to that from the control A549 cells. We used microarrays to detail the global program of gene expression controlled by Nkx2-1 and identified distinct classes of up-regulated and down-regulated genes. Overall design: mRNAs were isolated from control and Nkx2-1-induced A549 cells and hybridized on Affymetrix microarrays. We sought to identify genes influenced by Nkx2-1.

转录因子Nkx2-1（亦称TTF-1）可调控多组基因的表达。本研究以Nkx2-1诱导的A549细胞与对照组A549细胞的mRNA为实验材料开展基因表达分析。我们通过微阵列（microarrays）技术解析了Nkx2-1调控的全局基因表达程序，并鉴定出不同类别的上调基因与下调基因。总体实验设计：从对照组与Nkx2-1诱导的A549细胞中分离mRNA，将其与Affymetrix微阵列进行杂交。本研究旨在鉴定受Nkx2-1影响的基因。