A Peptidisc-based Survey of the Plasma Membrane Proteome of a Mammalian Cell

The low abundance and hydrophobicity of plasma membrane proteome relative to soluble proteins makes it difficult to characterize. Here, we apply the peptidisc membrane mimetic to purify the cell membrane proteome. We used HeLa cells as a reference to establish the method, and compared the plasma membrane proteomes between Pancreatic cancer cell Panc-1 and hPSC.

相较于可溶性蛋白，细胞膜蛋白质组（plasma membrane proteome）丰度较低且具有疏水性，其表征难度较大。本研究采用肽盘（peptidisc）膜模拟剂纯化细胞膜蛋白质组，以海拉细胞（HeLa）为参照建立该实验方法，并对比了胰腺癌细胞系Panc-1与人类多能干细胞（hPSC）的细胞膜蛋白质组。