Sequencing of the NS12911 (Venter) genome with mate-pair and linked-read WGS

We have conducted extensive sequencing on a well-characterized genome in order to aid development of new methodology for high-throughput sequencing analysis. Specifically, we have sequenced an extensive set of of Illumina libraries prepared from the NS12911 (Venter) genome: 2 kb, 5 kb, and 12 kb mate-pair libraries, and a 10X Genomics linked-read library (at 70x coverage). In combination with our previously-released short-insert Illumina data sets (200 bp and 350 bp inserts at 40x and 100x sequence coverage, respectively) and the existing Sanger sequencing experiments from this sample, our data can be used as a control/benchmark for new and existing tools for variant calling, haplotype phasing, and assembly.本研究针对一株已完成充分表征的基因组开展大规模测序，旨在助力高通量测序分析（high-throughput sequencing analysis）相关新方法的开发。具体而言，我们对源自NS12911（文特尔）基因组的多套Illumina测序文库（Illumina library）进行了测序：涵盖2 kb、5 kb及12 kb的Mate Pair文库（Mate Pair library），以及一套测序深度达70×的10X Genomics连锁读长文库（10X Genomics linked-read library）。结合本团队此前公开的短插入片段Illumina数据集（插入片段长度分别为200 bp与350 bp，测序深度各为40×及100×），以及该样本已有的Sanger测序（Sanger sequencing）实验数据，本数据集可作为现有及新型变异检测（variant calling）、单倍型分型（haplotype phasing）与基因组组装（genome assembly）工具的对照/基准测试数据集。