B_rapa_microbes_1

Finding differentially expressed genes in response to soil microbe treatments in two Brassica rapa genotypes--FPsc and R500. One week after germination seedlings were thinned to three plants per pot that were arranged in a ~6 cm equilateral triangle. Fourteen days later above- and below-ground plant tissue was collected from a single pot of each treatment from six spatial blocks to explore variation in gene expression across treatments. Leaf discs and root samples were placed into microcentrifuge tubes, snap frozen, and stored at -80 C until RNA extractions for transcriptomic profiling.

本数据集旨在探究两种芸薹（Brassica rapa）基因型FPsc与R500响应土壤微生物处理的差异表达基因（differentially expressed genes）。种子萌发一周后，对幼苗进行间苗，每盆保留3株植株，按约6厘米边长的等边三角形排布。14天后，从6个空间区组的各处理组单盆植株中采集地上与地下植物组织，以分析不同处理间的基因表达变异。将叶盘与根系样品置于微量离心管中，快速冷冻后于-80℃保存，直至开展用于转录组分析（transcriptomic profiling）的RNA提取工作。