Characterization of chicken peripheral blood mononuclear cells (PBMCs) during AIV prime vaccinations at single-cell resolution

We performed scRNA-seq for PBMCs isolated from 35-day old healthy male SPF chicken at the onset or upon vaccination with live-attenuated or inactivated AIV vaccines. Overall design: PBMC samples freshly isolated from 35-day-old healthy male SPF chickens were subjected to scRNA-seq using the 10 × Chromium platform.

本研究对35日龄健康雄性无特定病原体（specific pathogen free, SPF）鸡，在禽流感病毒（Avian Influenza Virus, AIV）减毒活疫苗或灭活疫苗接种的初始阶段及接种后分离的外周血单个核细胞（peripheral blood mononuclear cell, PBMC）开展了单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）。总体实验设计：从35日龄健康雄性SPF鸡新鲜分离的PBMC样本，采用10× Chromium平台完成单细胞RNA测序。